Photodynamic Diagnosis Using 5-Aminolevulinic Acid for the Detection of Positive Surgical Margins during Radical Prostatectomy in Patients with Carcinoma of the Prostate: A Multicentre, Prospective, Phase 2 Trial of a Diagnostic Procedure.
Author(s): Adam C, Salomon G, Walther S, Zaak D, Khoder W, Becker A, Reich O, Blana A, Ganzer R, Denzinger S, Popken G, Sroka R, Knüchel-Clarke R, Köllermann J, Sauter G, Hartmann A, Bertz S, Graefen M, Huland H, Wieland W, Stief CG.
Surgical margin status after radical prostatectomy (RP) is a significant risk factor for tumour recurrence.
It is an intriguing concept to find a fluorescence marker for photodynamic diagnosis (PDD) to make tumour margins visible during surgery.
OBJECTIVE: To investigate the feasibility of identification of positive surgical margins (PSM) during open retropubic or endoscopic extraperitoneal
RP by 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) to enhance surgical radicality. DESIGN, SETTING, AND PARTICIPANTS: Thirty-nine
patients (Gleason score 6-10, prostate-specific antigen [PSA] 2.3-120ng/ml) received 20mg/kg of body weight of 5-ALA orally and underwent RP
(24 endoscopic extraperitoneal, 15 open retropubic). MEASUREMENTS: A PDD-suitable laparoscopy optic (Karl-Storz GmbH, Tuttlingen, Germany) with a
yellow long-pass filter was coupled to a fibre-optic light cord with an excitation light source (380-420nm, D-Light, Karl-Storz GmbH, Tuttlingen,
Germany) for fluorescence excitation of PpIX and to a PDD-suitable camera for video and photo documentation by the AIDA DVD system (Karl-Storz GmbH,
Tuttlingen, Germany). RESULTS AND LIMITATIONS: There were more false-negative cases in the open group (four vs two) than in the endoscopic group but
more false-positive cases in the endoscopic group (two vs none) than in the open group. The overall sensitivity and specificity were 54% and 91.6%,
respectively. The sensitivity of the endoscopic cases was much higher (75% vs 44%) than for the open cases, while the specificity was higher for the
open group (88.2% vs 100%). CONCLUSIONS: PDD with 5-ALA-induced PpIX during RP might be a feasible and effective method for reducing the rate of PSM.
The technique seems to be more practicable during endoscopic RP rather than open RP. Further clinical studies with higher patient volumes and further
development of the technique seem justified. TRIAL REGISTRATION: EudraCT: 2005-004406-93.
Photodynamic Selectivity of 5-Aminolevulinic Acid to Prostate Cancer Cells
Author(s): Sultan M. Sultan, M.D.; Abdel-Alim M. El-Doray, M.D.; Alfons Hofstetter, M.D.; Osama Abdel-Gawad, M.D.; Alaa El-Deen El-Mahdy, M.D. and Wael Khoder, M.Sc.
To determine the selectivity of 5 - aminolevulinic acid (5-ALA) as a photosensitizer to malignant prostatic cells in
men undergoing radical retropubic prostatectomy.